raw264 7 cell culture medium  (Elabscience Biotechnology)

Journal: Journal of Nanobiotechnology

Article Title: pH-responsive and dual-dynamically crosslinked metal-phenolic hydrogel for synergistic macrophage and Th17/Treg reprogramming in diabetic wounds

doi: 10.1186/s12951-025-03987-7

Figure Lengend Snippet: Biocompatibility and hemostatic properties of CSp-OxD@Cu 2+ /G hydrogel. A-B) Live/dead staining images and statistics for HUVEC s across treatment groups; C-D) Live/dead staining images and statistics for RAW264.7 cells across treatment groups; E) H&E staining of mouse heart, kidney, liver, lung, and spleen across treatment groups; F-G) Hemostatic capacity and blood loss data for CSp-OxD@Cu 2+ /G hydrogel. Data are presented as mean ± SD, n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001, and ns, not significant

Article Snippet: The RAW264.7 cells were grown in an incubator set to 37 °C with 5% CO 2 , utilizing a specialized RAW264.7 cell culture medium from Servicebio, China.

Techniques: Staining

Journal: Journal of Nanobiotechnology

Article Title: pH-responsive and dual-dynamically crosslinked metal-phenolic hydrogel for synergistic macrophage and Th17/Treg reprogramming in diabetic wounds

doi: 10.1186/s12951-025-03987-7

Figure Lengend Snippet: CSp-OxD@Cu 2+ /G hydrogel influences macrophage polarization and inflammatory factor expression. A ) Effect of different concentrations of guaiacol on RAW264.7 cell polarization; Hydrogel’s role in regulating B ) RAW264.7 cell polarization and C) inflammatory factor expression. Data are presented as mean ± SD, n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001, and ns, not significant

Article Snippet: The RAW264.7 cells were grown in an incubator set to 37 °C with 5% CO 2 , utilizing a specialized RAW264.7 cell culture medium from Servicebio, China.

Techniques: Expressing

Journal: Journal of Nanobiotechnology

Article Title: pH-responsive and dual-dynamically crosslinked metal-phenolic hydrogel for synergistic macrophage and Th17/Treg reprogramming in diabetic wounds

doi: 10.1186/s12951-025-03987-7

Figure Lengend Snippet: CSp-OxD@Cu 2+ /G hydrogel affects macrophage pyroptosis and energy metabolism. A ) Western blot analysis, B ) Relative ATP levels, C ) ATP source proportions, D ) Glycolysis-related mRNA expression and E ) JC-1 staining results of RAW264.7 under various treatments. Data are presented as mean ± SD, n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001, and ns, not significant

Article Snippet: The RAW264.7 cells were grown in an incubator set to 37 °C with 5% CO 2 , utilizing a specialized RAW264.7 cell culture medium from Servicebio, China.

Techniques: Western Blot, Expressing, Staining

Journal: Journal of Nanobiotechnology

Article Title: pH-responsive and dual-dynamically crosslinked metal-phenolic hydrogel for synergistic macrophage and Th17/Treg reprogramming in diabetic wounds

doi: 10.1186/s12951-025-03987-7

Figure Lengend Snippet: Metabolic effects of CSp-OxD@Cu 2+ /G hydrogel on macrophages. A-D ) Metabolomics analysis of RAW264.7 cells (LPS, LPS + CSp-OxD@Cu 2+ /G), including A ) principal component analysis, B) volcano plot of differential metabolites, C ) clustering heatmap of metabolites, and D ) KEGG enrichment analysis; E ) Relative intracellular and extracellular uric acid levels in RAW264.7 cells under different treatments; F ) CD4 + T cell inflammatory factor expression after varying uric acid concentrations. Data are presented as mean ± SD, n = 6; * P < 0.05, ** P < 0.01, *** P < 0.001, and ns, not significant

Article Snippet: The RAW264.7 cells were grown in an incubator set to 37 °C with 5% CO 2 , utilizing a specialized RAW264.7 cell culture medium from Servicebio, China.

Techniques: Expressing